Characterization of New Mutants in the 16s Ribosomal Subunit Region of the Mitochondrial Genome of Saccharomyces Cerevisiae

............................................................................. ix Introduction ......................................................................... 1 Yeast Life Cycle............................................................. 2 Mitochondria..................................................................4 Nuclear vs. Mitochondrial Inheritance....................................5 Yeast Mitochondrial Mutants...............................................6 Yeast Mitochondria..........................................................8 Ribosomes.....................................................................9 Mitochondrial Ribosomes...................................................10 Var 1.....................................................................................................12 Antibiotic Resistance and the Ribosomal Region.........................12 The 16S rRNA gene..........................................................18 Paromomycin..................................................................18 Mutagenesis...................................................................22 Genetic Mapping..............................................................23 The Phage Analogy Model..................................................24 Project Objectives............................................................24